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"CD74-ROS1", "fusion_support_fraction": 0.080163, "fusionsieve_version": "1.1.0.dev0", "hard_filters": [], "integrity_verified": true, "left_error_bp": 2, "pairs_added": 75, "planted_mapped_primary_clipped_fraction": 0.646667, "planted_mapped_primary_clipped_records": 97, "planted_mapped_primary_records": 150, "planted_primary_mapq_max": 60, "planted_primary_mapq_mean": 59.986667, "planted_primary_mapq_median": 60.0, "planted_primary_mapq_min": 58, "planted_primary_records": 150, "planted_qnames_expected": 75, "planted_records_total": 223, "planted_secondary_records": 73, "planted_supplementary_records": 0, "planted_tag_AS_mapped_primary_count": 150, "planted_tag_AS_mapped_primary_fraction": 1.0, "planted_tag_MD_mapped_primary_count": 150, "planted_tag_MD_mapped_primary_fraction": 1.0, "planted_tag_NM_mapped_primary_count": 150, "planted_tag_NM_mapped_primary_fraction": 1.0, "planted_tag_RG_mapped_primary_count": 150, "planted_tag_RG_mapped_primary_fraction": 1.0, "planted_tag_SA_mapped_primary_count": 73, "planted_tag_SA_mapped_primary_fraction": 0.486667, "planted_tag_XS_mapped_primary_count": 150, "planted_tag_XS_mapped_primary_fraction": 1.0, "planted_unmapped_primary_records": 0, "planted_unmapped_records": 0, "planted_unmapped_secondary_records": 0, "planted_unmapped_supplementary_records": 0, "right_error_bp": 2, "score": 1.0, "soft_filters": [ "auto_reporting_disabled", "unvalidated_scorer", "missing_pon", "unverified_duplicate_processing" ], "spanning_pairs": 11, "spanning_pairs_planted": 15, "spanning_pairs_unique": 11, "spiked_all_candidates": 228, "spiked_gene_pair_candidates": 1, "spiked_primary_bam_records": 884382, "spiked_target_window_matches": 1, "split_fragments_planted": 60, "split_read_ends_planted": 98, "split_reads": 80, "split_reads_unique": 52, "target_candidate_found": true, "target_detected": true, "target_reportable": false, "target_window_ambiguous": false, "target_window_bp": 5, "tier": "REVIEW", "total_support": 63 } ], "created_utc": "2026-07-31T22:24:43.841981+00:00", "integrity": { "alignment_manifests_verified": 14, "all_referenced_file_sizes_and_sha256_verified": true, "controls_verified": 7, "fusionsieve_rerun_manifests_verified": 14, "fusionsieve_result_json_verified": 14, "matched_baselines_with_zero_target_window": 7, "private_sample_or_read_group_identifiers_exported": false, "tag_audits_verified": 14 }, "interpretation": { "detection": "Exactly one spiked target-window candidate in REVIEW, PASS, or HIGH with no hard filters, and zero matched-baseline window calls.", "reportability": "Automatic reportability is stricter: the detected candidate must be PASS or HIGH. REVIEW is detection/review evidence, not an automatically reportable result.", "tags": "AS, NM, MD, and RG are expected on mapped primary records. XS is conditional on a suboptimal alignment; SA is conditional on a chimeric/split representation." }, "schema": "spikeforge-full-reference-aggregate-v1", "scientific_scope": "BWA-MEM replay from pair-preserving shuffled FASTQs reconstructed from post-processed BAMs, followed by matched FusionSieve BAM runs; not a recreation of unavailable instrument FASTQs or the historical Picard/GATK post-processing chain.", "status": "PASS", "target_window": { "bp": 5, "definition": "Both gene-oriented breakpoints, chromosomes, and strands must match locked truth within +/-5 bp; reciprocal call orientation is normalized before signed errors are calculated.", "matched_baseline_requirement": "zero target-window candidates" } }